产品货号：
GS4879
中文名称：
重组人HMGB1(带His标签)
英文名称：
Recombinant Human High-Mobility Group Box 1, His
产品规格：
10μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 26.0kDa,a single non-glycosylated polypeptide chain containing 223 amino acids with 6×His at C-terminus.
储存条件：冷冻（-20℃）
概述
Human High-mobility group box 1 protein(HMGB1),previously known as HMG-1 or amphoterin,is a member of the high mobility group box family of non-histone chromosomal proteins.Human HMGB1 is expressed as a 30kDa,215 amino acid(a.a.)single chain polypeptide containing three domains:two N-terminal globular,70 a.a.positively charged DNA-binding domains(HMG boxes A and B),and a negatively charged 30 a.a.C-terminal region that contains only Asp and Glu.4,5 Residues 27 - 43 and 178 - 184 contain a NLS.Posttranslational modifications of the molecule have been reported,with acetylation occurring on as many as 17 lysine residues.HMGB1 is expressed at high levels in almost all cells.It was originally discovered as a nuclear protein that could bend DNA.Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人HMGB1(带His标签)，HMGB1, His-tagged, Human，Recombinant Human High-Mobility Group Box 1, His
储存条件：冷冻（-20℃）
概述
Human High-mobility group box 1 protein(HMGB1),previously known as HMG-1 or amphoterin,is a member of the high mobility group box family of non-histone chromosomal proteins.Human HMGB1 is expressed as a 30kDa,215 amino acid(a.a.)single chain polypeptide containing three domains:two N-terminal globular,70 a.a.positively charged DNA-binding domains(HMG boxes A and B),and a negatively charged 30 a.a.C-terminal region that contains only Asp and Glu.4,5 Residues 27 - 43 and 178 - 184 contain a NLS.Posttranslational modifications of the molecule have been reported,with acetylation occurring on as many as 17 lysine residues.HMGB1 is expressed at high levels in almost all cells.It was originally discovered as a nuclear protein that could bend DNA.Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞95%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Data not available. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
| 内毒素 | Less than 1 EU/μg of rHuHMGB1,His as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH |
相关搜索：重组人HMGB1(带His标签)，HMGB1, His-tagged, Human，Recombinant Human High-Mobility Group Box 1, His